Purification and Characterization of Protease from Bacillus thuringiensis Isolated from Soil
نویسندگان
چکیده
منابع مشابه
Production and purification of a protease from an alkalophilic Bacillus sp. 2-5 strain isolated from soil
This research has focused on isolation and characterization of a strain of Bacillus <span style="font-variant: normal; font-style: normal; font-family: ArialMT...
متن کاملPartial Purification and Characterization of Alkaline Protease from Bacillus sp. Isolated from Soil
Bacillus isolates were analyzed for alkaline protease production on casein containing agar plates and identified by clear zones of casein hydrolysis around colonies. Two gram positive Bacillus isolates S5 and C3 which showed the best enzyme production were studied for purification by precipitation and dialysis. The intracellular alkaline protease enzyme was purified about 4 fold with a yield of...
متن کاملIsolation, Purification and Characterization of a Thermophilic Alkaline Protease from Bacillus subtilis BP-36
The goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native Iranian microorganisms. At the end of screening program, a Bacillus subtilis BP-36 strain producing thermophilic alkaline protease was isolated from a hot spring in Ardebil province. The target enzyme was purified using a one-step Aqueous two-phase systems (ATPS) prot...
متن کاملPectinase production and purification from Bacillus subtilis isolated from soil
Pectinases are industrially important enzymes. The aim of this work was the isolation of pectinases producing Bacillus species from soil sample and their identification by phenotypic tests as well as production and purification pectinases enzyme. Isolated bacteria Subjected to various screening activity and gives pectinases screening test positive i.e. bacteria produced pectinases enzyme. The e...
متن کاملproduction and purification of a protease from an alkalophilic bacillus sp. 2-5 strain isolated from soil
this research has focused on isolation and characterization of a strain of bacillus sp. from alkaline soil, which was able to produce extracellular alkaline protease at phs ranging from 8 to 11 and temperatures of 20 to 50ºc. also the impact of different carbon andnitrogen sources were investigated. the yield and fold of enzyme purification was 24% and 50 times, respectively. molecular weight o...
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ژورنال
عنوان ژورنال: International Journal of Science Letters
سال: 2021
ISSN: 2687-4733
DOI: 10.38058/ijsl.842485